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. 2020 Oct 14;9:e60547. doi: 10.7554/eLife.60547

Video 5. Neutrophil endothelial rolling within a post-capillary venule confirmed using fluorescent labeling with anti-Ly6G antibody.

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Representative example of retinal vein imaged six hours post LPS injection. Simultaneous aligned acquisition of 796 nm phase contrast (top panel) and anti-Ly6G conjugated AlexaFluor 647 (positively labeling neutrophils in bottom panel) using confocal AOSLO fluorescence. Scale bar = 10 μm.