Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference |
Identifiers | Additional information |
|---|---|---|---|---|
| Cell line (Homo-sapiens) | MRC5 primary fetal lung fibroblasts | Purchased from ATCC | CCL-17 | |
| Cell line (Homo-sapiens) | ARPE-19 retinal pigment epithelial cells | Purchased from ATCC | CRL-2302 | |
| Cell line (Homo-sapiens) | Phoenix-AMPHO kidney epithelial cells | Purchased from ATCC | CRL-3213 | |
| Strain, strain background (Human Cytomegalovirus) | HCMV BAC clone | Thomas Shenk (Princeton University) (Yu et al., 2002) | AD169 | |
| Strain, strain background (Human Cytomegalovirus) | HCMV BAC clone |
Richard Stanton (Cardiff University) (Stanton et al., 2010) | RCMV1158 | Unrepaired Merlin virus with GFP cassette |
| Strain, strain background (Human Cytomegalovirus) | HCMV BAC clone | Richard Stanton (Cardiff University) (Stanton et al., 2010) | RCMV1120 | UL128 repaired Merlin virus without tags |
| Recombinant DNA reagent | pLXSN | Clontech | 631509 | Retroviral construct to transduce and express VAMP3 |
| Sequence-based reagent | vamp3_F | This paper | PCR primer | 5’-TGACGAATTCATGTCTACAGGTCCA-3’ |
| Sequence-based reagent | vamp3_R | This paper | PCR primer | 5’-TGAGGATCCTCATGAAGAGACAAC-3’ |
| Sequence-based reagent | ul83_F | Gault et al., 2001 | qPCR primer | 5’-GTCAGCGTTCGTGTTTCCCA-3’ |
| Sequence-based reagent | ul83_R | Gault et al., 2001 | qPCR primer | 5’-GGGACACAACACCGTAAAGC-3’ |
| Sequence-based reagent | atg5_F | This paper | qPCR primer | 5’-TATTACCCTTTGATGCCTTTTTTTC-3’ |
| Sequence-based reagent | atg5_R | This paper | qPCR primer | 5’-AACTTGTACCACCAATTCTAAAATG-3’ |
| Sequence-based reagent | siRNA screen qPCR primers | This paper | All sequences available in Supplementary file 3 | |
| Sequence-based reagent | siGENOME SMARTpool siRNA | Dharmacon | All catalogue numbers available in Supplementary file 3 | All sequences available in Supplementary file 3 |
| Antibody | anti-human CD63 (Mouse monoclonal) | Santa Cruz | sc-5275 | WB (1:1000) |
| Antibody | anti-human CD81 (Mouse monoclonal) | Santa Cruz | sc-166029 | WB (1:1000) |
| Antibody | anti-human VPS4 (Mouse monoclonal) | Sigma | SAB4200215 | WB (1:1000) |
| Antibody | anti-human TSG101 (Mouse monoclonal) | GeneTex | GTX70255 | WB (1:1000) |
| Antibody | anti-human ALIX/PDCD6IP (Mouse monoclonal) | Cell Signalling Technology | #2171 | WB (1:1000) |
| Antibody | anti-HCMV gB (Mouse monoclonal) | Abcam | ab6499 | WB (1:1000) IF (1:500) |
| Antibody | anti-HCMV UL83 (Mouse monoclonal) | Thomas Shenk (Princeton University) Nowak et al., 1984 | Clone 8F5 | WB (1:1000) |
| Antibody | anti-HCMV UL99 (Mouse monoclonal) | Thomas Shenk (Princeton University) Silva et al., 2003 | Clone 10B4 | WB (1:1000) IF (1:500) |
| Antibody | anti-human β-actin (Mouse monoclonal) | Sigma | A2228 | WB (1:1000) |
| Antibody | anti-human VAMP3 (Rabbit monoclonal) | Abcam | ab200657 | WB (1:1000) IF (1:500) |
| Antibody | anti-HCMV IE1 (Mouse monoclonal) | Thomas Shenk (Princeton University) (Zhu et al., 1995) | Clone 1B12 | IF (1:100) |
| Antibody | anti-human GM130 (Mouse monoclonal) | BD Biosciences | 610822 | IF (1:500) |
| Antibody | anti-human GM130 (Rabbit monoclonal) | Abcam | ab52649 | IF (1:500) |
| Antibody | goat anti-mouse Alexa Fluor (568) | Invitrogen | A-11004 | IF (1:1000) |
| Antibody | goat anti-rabbit Alexa Fluor (633) | Invitrogen | A-21070 | IF (1:1000) |
| Antibody | goat anti-mouse HRP conjugate | Bio-Rad | #1706516 | WB (1:5000) |
| Antibody | goat anti-rabbit HRP conjugate | Bio-Rad | #1706515 | WB (1:5000) |
| Commercial assay or kit | Viral DNA spin kit | Qiagen | Cat No./ID: 57704 | |
| Software, algorithm | MaxQuant | MaxQuant (Cox and Mann, 2008) | version 1.6.0.13 | |
| Software, algorithm | Perseus | Perseus (Tyanova et al., 2016) | version 1.6.0.7 | |
| Software, algorithm | GOPlot | GOPlot (Walter et al., 2015) | v.1.0.2, CRAN |