Appendix 1—key resources table.
| Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Genetic reagent Mus musculus |
PomkLoxP/LoxP ICR | This paper | Campbell Lab | Materials and Methods ‘Generation of PomkLoxP/LoxP Mice’ |
| Genetic reagent Mus musculus |
Pax7Cre
C57BL/6J |
The Jackson Laboratory, Bar Harbor ME. | JAX:010530, RRID:IMSRJAX:010530 | Pax7tm1(cre)Mrc |
| Genetic reagent Mus musculus |
MckCre
C57BL/6J |
The Jackson Laboratory, Bar Harbor ME. | JAX:006475, RRID:IMSR_JAX:006475 | B6.FVB(129S4)-Tg(Ckmm-cre)5Khn/J |
| Genetic reagent Mus musculus |
Largemyd
C57BL/6J |
The Jackson Laboratory, Bar Harbor ME. | JAX:000300, RRID:IMSR_JAX:000300 | MYD/Le-Os +/+ Largemyd/J myd |
| Antibody | anti-DG (Sheep polyclonal) |
R and D Systems | Cat# AF6868, RRID:AB_10891298 |
WB (1:500) |
| Antibody | anti-α-DG (IIH6C4) (Mouse monoclonal) |
DSHB Campbell Lab |
Cat# IIH6 C4, RRID:AB_2617216 |
WB (1:10-1:100) |
| Antibody | anti-myc clone 4A6 (Mouse monoclonal) |
Millipore | Cat# 05–724, RRID:AB_309938 |
WB (1:2000) |
| Antibody | anti-α-DG (IIH6C4) (Mouse monoclonal) |
Millipore Campbell Lab |
Cat# 05–593, RRID:AB_309828 |
IF (1:1000-1:2000) |
| Antibody | anti-Laminin (Rabbit polyclonal) | Sigma-Aldrich | Cat# L9393, RRID:AB_477163 | WB (1:1000), Solid-Phase Assay (1:5000) |
| Antibody | anti-β-DG (Rabbit polyclonal) |
Campbell Lab PMID:1741056 DOI:10.1038/355696a0 |
AP83 | IF (1:50) |
| Antibody | anti-β-DG mouse IgM (Mouse monoclonal) |
Leica Biosystems | Cat# NCL-b-DG, RRID:AB_442043 | IF (1:50 to 1:200) |
| Antibody | anti-Na+,K+ ATPase (Mouse monoclonal) |
BD Biosciences | Cat# 610993 RRID:AB_398306 |
WB (1:1000) |
| Antibody | anti-sheep IgG (Donkey polyclonal) |
Rockland | Cat# 613-731-168, RRID:AB_220181 | WB (1:2000) |
| Antibody | anti-mouse IgG (H + L) (Donkey polyclonal) |
LI-COR Biosciences | Cat# 926–32212, RRID:AB_621847 | WB (1:15,000), IF (1:800) |
| Antibody | anti-rabbit IgG (H + L) (Donkey polyclonal) |
LI-COR Biosciences | Cat# 926–32213, RRID:AB_621848 | WB (1:15,000), IF (1:800) |
| Antibody | anti-mouse IgM (Goat polyclonal) |
LI-COR Biosciences | Cat# 926–32280, RRID:AB_2814919 | WB (1:2500) |
| Antibody | anti-mouse IgG1 (Goat polyclonal) |
LI-COR Biosciences | Cat# 926–32350, RRID:AB_2782997 | WB (1:2000, 1:10,000) |
| Antibody | anti-rabbit IgG (H+L) (Goat polyclonal) |
Thermo Fisher Scientific | Cat# A-11034, RRID:AB_2576217 | IF (1:1000 to 1:2000) |
| Antibody | anti-mouse IgM (Goat polyclonal) | Thermo Fisher Scientific | Cat# A-21042, RRID:AB_2535711 | IF (1:1000 to 1:2000) |
| Antibody | anti-human FLJ23356 (Mouse monoclonal) |
Novus | Cat# H00084197-M03, RRID:AB_2188284 | WB (1:500) |
| Commercial assay or kit | Creatine Kinase (CK) Liqui-UV Test | Fisher Scientific/Stanbio | Cat# 22-022-630 | |
| Cell line (Homo-sapiens) |
Parental cell line C631 | Horizon Discovery | Cat# C631 | Mycoplasma testing passed |
| Cell line (Homo-sapiens) |
POMK/DAG1 KO | Horizon Discovery | HZGHC001338c004, RRID:CVCL_TF19 | Authenticated by Sanger sequencing. Mycoplasma testing passed. |
| Cell line (Homo-sapiens) |
POMK/LARGE1 KO | Horizon Discovery | HZGHC007364c011 | Authenticated by Sanger sequencing. Mycoplasma testing passed. |
| Cell line (Homo-sapiens) |
POMK KO | Horizon Discovery | HZGHC001338c004, RRID:CVCL_TF19 | Authenticated by Sanger sequencing. Mycoplasma testing passed. |
| Cell line (Homo-sapiens) |
POMK/ISPD KO | Horizon Discovery | HZGHC001338c001, RRID:CVCL_TF18 | Authenticated by Sanger sequencing. Mycoplasma testing passed. |
| Cell line (Homo-sapiens) |
FKTN KO | Horizon Discovery | HZGHC000721c010, RRID:CVCL_SN68 | Authenticated by Sanger sequencing. Mycoplasma testing passed. |
| Cell line (Homo-sapiens) |
LARGE1 KO | Horizon Discovery | HZGHC000122c007, RRID:CVCL_SV31 | Authenticated by Sanger sequencing. Mycoplasma testing passed. |
| Cell line (Homo-sapiens) |
Primary dermal fibroblasts, human | ATCC | PCS-201–012 | |
| Cell line (Homo-sapiens) |
Human fibroblasts (POMK D204N) | This paper | NH13-284 | Dubowitz Neuromuscular Center, Campbell Lab |
| Peptide, recombinant protein | β-Glucuronidase | PMID:16303119
DOI:10.1016/j.carres.2005.10.005 |
||
| Peptide, recombinant protein | α-Xylosidase | PMID:10801892
DOI:10.1074/jbc.M910392199 |
||
| Biological sample (Homo-sapiens) | Control human skeletal muscle | This paper | Dubowitz Neuromuscular Center, Campbell Lab | |
| Biological sample (Homo-sapiens) | Human skeletal muscle | This paper | (NH13-284, POMK D204N) | Dubowitz Neuromuscular Center, Campbell Lab |
| Chemical compound, drug | Purified Danio rerio POMK | PMID:27879205
DOI:10.7554/eLife.22238 |
||
| Chemical compound, drug | Purified mammalian dTMLARGE1 | PMID:22223806
DOI:10.1126/science.1214115 |
||
| Chemical compound, drug | GGM-MU and GGMp-MU | PMID:23929950
DOI:10.1126/science.1239951 |
||
| Chemical compound, drug | UDP-Xylose | CarboSource | https://www.ccrc.uga.edu/~carbosource/CSS_substrates.html | |
| Chemical compound, drug | 4-Methylumbelliferyl-β-D-xylopyranoside | Sigma/Millipore | Cat# M7008 | |
| Chemical compound, drug | UDP-Glucuronic acid | Sigma/Millipore | Cat# U6751 | |
| Chemical compound, drug | Uridine 5′-diphospho-N-acetylgalactosamine disodium salt | Sigma/Millipore | Cat# U5252 | |
| Chemical compound, drug | Uridine 5′-diphospho-N-acetylglucosamine sodium salt | Sigma/Millipore | Cat# U4375 | |
| Chemical compound, drug | 4-methylumbelliferyl α-(GlcNAc-β(1-4)Man) GM-MU | Sussex Research | https://www.sussex-research.com/ | |
| Chemical compound, drug | Xylose-α1,3-GlcA-β-MU | Sussex Research | https://www.sussex-research.com/ | |
| Chemical compound, drug | Pepstatin A | Sigma/Millipore | Cat# 516481 | |
| Chemical compound, drug | Calpain Inhibitor I (25 mg) | Sigma/Millipore | Cat# A6185 | |
| Chemical compound, drug | Aprotinin from bovine lung | Sigma/Millipore | Cat# A1153 | |
| Chemical compound, drug | Leupeptin (25 mg) | Sigma/Millipore | Cat# 108975 | |
| Chemical compound, drug | PMSF | Sigma/Millipore | Cat# P7626-25G | |
| Chemical compound, drug | Immobilon-FL PVDF | Sigma/Millipore | Cat# IPFL00010 | |
| Chemical compound, drug | Calpeptin | Fisher Scientific | Cat# 03-340-05125M | |
| Chemical compound, drug | Bis-acrylamide solution-30% (37:1) | Fisher Scientific/Hoefer | Cat# HBGR337500X | |
| Chemical compound, drug | Benzamidine Hydrochloride Hydrate | MP Biochemicals | Cat# 195068 | |
| Chemical compound, drug | WGA agarose bound | Vector Labs | Cat# AL-1023, RRID:AB_2336862 | |
| Chemical compound, drug | Precision Plus Protein All Blue Standards-500ul | Bio-Rad | Cat# 161–0373 | |
| Software, algorithm | SigmaPlot | SigmaPlot | RRID:SCR_003210 | |
| Software, algorithm | Excel | Microsoft | RRID:SCR_016137 | |
| Software, algorithm | GraphPad Prism | https://www.graphpad.com/scientific-software/prism/ | RRID:SCR_002798 | Version 8.3 |
| Software, algorithm | FlowJo | https://www.flowjo.com/solutions/flowjo/downloads | RRID:SCR_008520 | Version 7.6.5 |
| Software, algorithm | Li-Cor Image Studio Software | https://www.licor.com/bio/image-studio-lite/download | RRID:SCR_015795 | |
| Other | Streptavidin, Alexa Fluor 594 conjugate | Thermo Fisher Scientific | Cat# S11227 | IF (1:1000 to 1:2000) |
| Other | Adenovirus: DGC (DG, delta H30-A316) |
PMID:21987822
DOI:10.1073/pnas.1114836108 |
||
| Other | Adenovirus: DG T317A | PMID:21987822
DOI:10.1073/pnas.1114836108 |
||
| Other | Adenovirus: DG T319A | PMID:21987822
DOI:10.1073/pnas.1114836108 |
||
| Other | Adenovirus: DG T317A/319A | PMID:21987822
DOI:10.1073/pnas.1114836108 |
||
| Other | Adenovirus: DG Wild-Type (WT) | PMID:21987822
DOI:10.1073/pnas.1114836108 |
||
| Other | Adenovirus: POMK WT |
PMID:27879205
DOI:10.7554/eLife.22238 |
||
| Other | Adenovirus: POMK D204A | PMID:27879205
DOI:10.7554/eLife.22238 |
||
| Other | Adenovirus: POMK D204N | This paper | Campbell Lab | Materials and methods ‘Adenovirus Production’ |
| Other | Adenovirus: DG390TEVHis | This paper | Campbell Lab | Materials and methods ‘Adenovirus Production’ |
| Other | Adenovirus: Fukutin | PMID:22522420
DOI:10.1038/ng.2252 |
||
| Other | Adenovirus: Isoprenoid Synthase Domain-Containing (ISPD) | PMID:22522420
DOI:10.1038/ng.2252 |
||
| Other | Adenovirus: LARGE1 | PMID:22522420
DOI:10.1038/ng.2252 |
||
| Other | NMR spectrometer | Bruker | Avance II 800 MHz | |
| Other | Rodent Treadmill | Columbus Instruments | Exer 3/6 Treadmill | |
| Other | Western Blot Imager | Li-Cor | Odyssey CLx | |
| Other | Mouse treadmill | Omnitech Electronics | Accupacer Treadmill | |
| Other | Isolated Mouse Muscle System | Aurora Scientific | 1200A | |
| Other | Mouse Grip Strength Meter | Columbus Instruments | 1027 Mouse | |
| Other | Prominence HPLC | Shimadzu | LC-20 system | |
| Other | Tabletop ultracentrifuge | Beckman Coulter | Optima max, 130K | |
| Other | Ultracentrifuge | Beckman Coulter | Optima-L-100 XP | |
| Other | Centrifuge | Beckman Coulter | Avanti J-E HPC | |
| Other | HPLC LC18 column | Supelco | 58368 |