For the locomotion-triggered averages here, instead of being normalized to the arteriole baseline for the vehicle condition, the baseline dimeter during the manipulation (e.g. CNO or drug) was used. Baseline changes can mask changes in evoked amplitude when measure ratiometrically. (A) muscimol, vehicle 15.8 ± 2.7%, muscimol 4.4 ± 0.3%, LME p<0.04, n = 6 mice. (B) hSyn-G(i) DREADDs, vehicle 20.5 ± 2.0%, CNO 17.8 ± 2.8%, LME p<1, n = 7 mice. (C) hSyn-G(q) DREADDs, vehicle 18.6 ± 1.7%, CNO 9.7 ± 2.1%, LME p<7.1×10−3, n = 7 mice. (D) CaMKIIa-G(i) DREADDs, vehicle 25.3 ± 1.9%, CNO 21.4 ± 3.0%, LME p<1, n = 7 mice. (E) CaMKIIa-G(q) DREADDs, vehicle 19.1 ± 2.3%, CNO 9.0 ± 2.1%, LME p<0.06, n = 6 mice. (F) nNOS-G(i) DREADDs, vehicle 15.3 ± 2.0%, CNO 9.8 ± 2.1%, LME p<0.26, n = 9 mice. (G) nNOS-G(q) DREADDs, vehicle 17.4 ± 1.8%, CNO 13.1 ± 1.8%, LME p<0.94, n = 6 mice. (H) L-NAME, vehicle 13.1 ± 2.3%, L-NAME 6.1 ± 2.2%, LME p<0.5, n = 6 mice. (I) CP-99994, vehicle 13.7 ± 2.6%, CP-99994 9.8 ± 2.1%, LME p<1, n = 7 mice. (J) Substance P, vehicle 13.0 ± 2.2%, Substance P 8.7 ± 2.3%, LME p<1, n = 7 mice. (K) Reporter virus, vehicle 17.7 ± 1.5%, CNO 18.3 ± 1.9%, LME p<1, n = 12 mice. (L) ML-133, vehicle 14.0 ± 3.0%, ML-133 6.0 ± 3.3%, LME p<1, n = 5 mice. (M) BaCl2, vehicle 15.1 ± 2.7%, BaCl211.1 ± 5.5%, LME p<1, n = 5 mice.