Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Strain, strain background (Mus musculus) |
C57BL/6J | Jackson Labs | #000664 | |
| Strain, strain background (Mus musculus) |
B6.129-Nos1tm1(cre)Mgmj/J | Jackson Labs | #017526 | |
| Strain, strain background (Mus musculus) |
FVB-Tg(Aldh1l1-cre/ERT2) 1Khakh/J |
Jackson Labs | #023748 | |
| Strain, strain background (Mus musculus) |
C57BL/6-Tg(CAG-EGFP) 131Osb/LeySopJ |
Jackson Labs | #006567 | |
| Genetic reagent (Adeno-associated virus) |
AAV5-CMV-Turbo RFP-WPRE-rBG |
UNC vector core | ||
| Genetic reagent (Adeno-associated virus) |
AAV8-hSYN-HA-hM3D (Gq)-mCherry |
Addgene | #50474-AAV8 | |
| Genetic reagent (Adeno-associated virus) |
AAV5-hSYN-HA-hM4D (Gi)- mCherry |
UNC Vector Core | ||
| Genetic reagent (Adeno-associated virus) |
AAV5-CaMKIIa-hM3D (Gq)- mCherry |
Addgene | #50476-AAV5 | |
| Genetic reagent (Adeno-associated virus) |
AAV5-CaMKIIa-hM4D (Gi)- mCherry |
UNC Vector Core | ||
| Genetic reagent (Adeno-associated virus) |
AAV8-DIO-hM3D (Gq)-mCherry |
Addgene | #44361-AAV8 | |
| Genetic reagent (Adeno-associated virus) |
AAV8-DIO-hM4D (Gi)-mCherry |
Addgene | #44362-AAV8 | |
| Genetic reagent (Adeno-associated virus) |
AAV9-CaMKII-GCaMP6s | Addgene | #107790-AAV9 | |
| Genetic reagent (Adeno-associated virus) |
AAV8-syn-GCaMP-WPRE | Vigene | AAV8-syn-GCaMP-WPRE | |
| Genetic reagent (Adeno-associated virus) |
AAV9-CaMKII-GCaMP6s | Addgene | #107790-AAV9 Viagene |
|
| Antibody | Mouse monoclonal GAD-65 Antibody (A-3) |
Santa Cruz Biotechnology | sc-377145 | (1:200) |
| Antibody | Mouse monoclonal CaMKII Antibody (G-1) |
Santa Cruz Biotechnology | sc-5306 | (1:200) |
| Antibody | Rabbit polyclonal to GFAP | Abcam | ab7260 | (1:200) |
| Antibody | Alexa Fluor 488 Goat Anti-Mouse IgG H and L |
Abcam | ab150113 | (1:500) |
| Antibody | Goat Anti-Rabbit IgG H and L | Abcam | ab6702 | (1:500) |
| Antibody | Goat polyclonal nNOS | Abcam | ab1376 | (1:200) |
| Chemical compound, drug | muscimol | Sigma-Aldrich | M1523 | 10 mM |
| Chemical compound, drug | L-NAME | Sigma-Aldrich | N5751 | 1 mM |
| Chemical compound, drug | Substance P | Tocris | 1156 | 1 mM |
| Chemical compound, drug | CP-99994 | Tocris | 3417 | 8 mM |
| Chemical compound, drug | ML-133 | Sigma-Aldrich | SML0190 | 10 µM |
| Chemical compound, drug | DMSO | Sigma-Aldrich | D8418 | 1% |
| Chemical compound, drug | BaCl2 | EMD Millipore | B1493816 | 100 µM |
| Chemical compound, drug | CNO | Sigma-Aldrich | C0832 | 2.5 mg/kg |
| Chemical compound, drug | FITC-dextran 70 kDa | Sigma-Aldrich | 46945 | 50 µL at 5% |
| Chemical compound, drug | TRITC dextran 70kD | Sigma-Aldrich | T1162 | 50 µL at 5% |
| Software, algorithm | Pial vessel dimeter measurements |
https://github.com/DrewLab/
Surface-Vessel-FWHM-Diameter |
||
| Software, algorithm | Penetrating vessel dimeter measurements |
https://github.com/DrewLab/
Thresholding_in_Radon_Space |
||
| Software, algorithm | Red blood cell velocity measurements |
https://github.com/
DrewLab/MCS_Linescan |
||
| Software, algorithm | 2 P microscope control software |
MCS, Sutter Instruments, Novato CA | ||
| Software, algorithm | MATLAB | Mathworks, Natick MA | ||
| Software, algorithm | Doric Neuroscience Studio | Doric Lenses, Quebec, Quebec Canada |