Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Strain, strain background (V. cholerae A1552) | Vc Rg | Yildiz and Schoolnik, 1999 | Rugose variant | |
| Strain, strain background (V. cholerae A1552) | Vc WT | Yildiz and Schoolnik, 1999 | Smooth wild-type variant | |
| Strain, strain background (V. cholerae A1552) | Vc Rg ΔvpsL | This study | In frame deletion of vpsL in rugose background obtained by matings of Vc Rg with S17 harboring deletion plasmid pFY_922 | |
| Strain, strain background (V. cholerae A1552) | Vc Rg ΔrbmA | This study | In frame deletion of rbmA in rugose background obtained by matings of Vc Rg with S17 harboring deletion plasmid pFY_113 | |
| Strain, strain background (V. cholerae A1552) | Vc Rg Δbap1 | This study | In frame deletion of bap1 in rugose background obtained by matings of Vc Rg with S17 harboring deletion plasmid pFY_330 | |
| Strain, strain background (V. cholerae A1552) | Vc Rg ΔrbmA pBADrbmA | This study | Vc Rg ΔrbmA harboring the plasmid pNUT1236 | |
| Strain, strain background (V. cholerae A1552) | Vc Rg Δbap1 pBADbap1 | This study | Vc Rg Δbap1 harboring the plasmid pBAD/Myc-His B | |
| Strain, strain background (V. cholerae N16961) | N16961 | Drescher et al., 2016 | Smooth wild-type variant | |
| Strain, strain background (V. cholerae C6706) | C6706 | Thelin and Taylor, 1996 | Smooth wild-typevariant | |
| Strain, strain background (P. aeruginosa) | PAO1 WT | Hickman et al., 2005 | ||
| Strain, strain background (P. aeruginosa) | PAO1 Rg | Rybtke et al., 2012 | In frame deletions of wspF | |
| Strain, strain background (P. aeruginosa) | PAO1 Rg Δpel | Rybtke et al., 2012 | In frame deletions of wspF, pelA genes | |
| Strain, strain background (P. aeruginosa) | PAO1 Rg Δpsl | Rybtke et al., 2012 | In frame deletions of wspF, pslBCD genes | |
| Strain, strain background (P. aeruginosa) | PAO1 Rg ΔcdrA | Rybtke et al., 2015 | In frame deletions of wspF, cdrA genes | |
| Cell line (Homo sapiens) | Caco-2 | ATCC | HTB-37 RRID:CVCL_0025 |
|
| Cell line (Canis) | MDCK | Sigma Aldrich | 84121903-1VL RRID:CVCL_0422 |
|
| Cell line (Mus musculus) | CMT-93 | ATCC | RRID:CCL-223
RRID:CVCL_1986 |
|
| Recombinant DNA reagent | pFY_113 (plasmid) | Berk et al., 2012 | Plasmid for generation of in-frame rbmA deletion mutants | |
| Recombinant DNA reagent | pFY_330 (plasmid) | Berk et al., 2012 | Plasmid for generation of in-frame bap1 deletion mutants | |
| Recombinant DNA reagent | pFY_922 (plasmid) | Fong et al., 2010 | Plasmid for generation of in-frame vpsL deletion mutants | |
| Recombinant DNA reagent | pNUT1236 (plasmid) | Hartmann et al., 2019 | Arabinose-inducible plasmid containing the coding region of rbmA | |
| Recombinant DNA reagent | pBAD/Myc-His B (plasmid) | Fong and Yildiz, 2007 | Arabinose-inducible plasmid containing the coding region of bap1 | |
| Chemical compound, drug | Lithium phenyl-2,4,6- trimethylbenzoylphosphinate (LAP) | Tokio Chemical Industries | ||
| Chemical compound, drug | PEGDA (MW 3400, 6000, 10000) | Biochempeg | ||
| Chemical compound, drug | PEGDA (MW 700) | Sigma-Aldrich | ||
| Software | Fiji | Fiji | ||
| Software | OriginPro | OriginLab Corporation | ||
| Software | MATLAB | Mathworks | ||
| Software | Imaris | Bitplane | ||
| Algorithm | 3D TFM | Toyjanova et al., 2014 | ||
| Other | SYTO9 stain | Invitrogen | S34854 | 10 µM |
| Other | CellTracker Orange CMRA stain | Invitrogen | C34551 | 10 µM |
| Other | Hoechst stain | Thermo Fischer Scientific | 62249 | 5 µg/ml |
| Other | Calcein-AM stain | Sigma Aldrich | 17783 | 5 µM |