Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Antibody | Anti-Gli3 (Goat polyclonal) | R and D Systems | Cat.#AF3690; RRID:AB_2232499 | (1:1000) |
| Antibody | Anti-dHand M-19 (Goat polyclonal) | Santa Cruz Biotechnology | Cat.#Sc-9409; RRID:AB_2115995 |
(1:1000) |
| Antibody | Anti-Flag M2 (Mouse monoclonal) | Sigma-Aldrich | Cat.#F1804; RRID:AB_262044 | (1:1000) |
| Cell line (M. musculus) | O9-1 cells | Ishii et al., 2012 | Gift from R. Lipinski Lab | |
| Cell line (E. coli) | BL21 cells | Promega | Cat.#L1195 | |
| Peptide, recombinant protein | E47L/Tcf3 | Purified protein, contact B. Gebelein | NP_001157619.1 | peptide, recombinant protein |
| Peptide, recombinant protein | Gli3DBD | This paper | NP_032156.2 (full protein) | Purified protein, inquiries should be addressed to B. Gebelein |
| Peptide, recombinant protein | Hand2 | This paper | NP_034532.3 | Purified protein, inquiries should be addressed to B. Gebelein |
| Peptide, recombinant protein | Human FGF basic | R and D Systems | Cat.# 233-FB-025 |
|
| Peptide, recombinant protein | Leukemia Inhibitory Factor (LIF) | Millipore | Cat.# ESG1106 | |
| Commercial assay or kit | Dual Luciferase Reporter Assay System | Promega | Cat.#E1910 | |
| Genetic reagent (M. musculus) | Gli2flox | PMID:16571625 | JAX stock # 007926; RRID:IMSR_JAX:007926 | Gift from A. Joyner, Memorial-Sloan-Kettering Cancer Center |
| Genetic reagent (M. musculus) | Gli3flox | PMID:18480159 | JAX stock # 008873; RRID:IMSR_JAX:008873 | |
| Genetic reagent (M. musculus) | Hand2flox | PMID:17075884 | JAX stock # 027727; RRID:IMSR_JAX:027727 | |
| Genetic reagent (M. musculus) | Wnt1-Cre: H2az2Tg(Wnt1-cre)11Rth | PMID:9843687 | MGI:2386570; RRID:IMSR_JAX:003829 | Gift from R. Stottmann |
| Genetic reagent (M. musculus) | Gli3tm1.1Amc/Grsr (3XFLAGbio) | PMID:24990743; 27146892 | JAX stock #026135 |
Generated by K.A. Peterson |
| Genetic reagent (M. musculus) | Hand23xFlag | PMID:25453830 | Generated by R. Zeller | |
| Recombinant DNA reagent | pGL3-promoter (plasmid) | Promega | Cat.#E1761 | Luciferase reporter |
| Recombinant DNA reagent | p3XFLAG-Gli3 (plasmid) | This paper | Full-length Mouse Gli3 cloned into p3XFlag backbone. Inquiries should be addressed to S. Brugmann | |
| Recombinant DNA reagent | p3XFLAG-Hand2 (plasmid) | This paper | Full-length Mouse Hand2 cloned into p3XFlag backbone. Inquiries should be addressed to S. Brugmann | |
| Sequence-based reagent | Gli2_Flox_F | This paper | PCR primers | AGG TCC TCT TAT TGT CAG GC; Inquiries should be addressed to S. Brugmann |
| Sequence-based reagent | Gli2_Flox_R | This paper | PCR primers | GAG ACT CCA AGG TAC TTA GC; Inquiries should be addressed to S. Brugmann |
| Sequence-based reagent | Gli3_Flox_F | This paper | PCR primers | GTC TGT AAC CAG ACG GCA CT; Inquiries should be addressed to S. Brugmann |
| Sequence-based reagent | Gli3_Flox_R | This paper | PCR primers | GAG AAT GTG TGA CTC CAT GC; Inquiries should be addressed to S. Brugmann |
| Sequence-based reagent | Hand2_Flox_F | JAX | PCR primers | ACT TGC TGA CTG GGT CCT TG; |
| Sequence-based reagent | Hand2_Flox_R | JAX | PCR primers | CTC GGC CTA GAG GAC ACT GA |
| Sequence-based reagent | Cre_F | This paper | PCR primers | GTCCCATTTA CTGACCGTAC ACC; Inquiries should be addressed to S. Brugmann |
| Sequence-based reagent | Cre_R | This paper | PCR primers | GTTATTCGGA TCATCAGCTA CACC; Inquiries should be addressed to S. Brugmann |
| Sequence-based reagent | 5’IREdye-700 labeled oligo | IDT | For EMSA assays | N/A |
| Sequence-based reagent | RNAscope probe- Mm Gli3 | Advanced Cell Diagnostics | Cat.#445511 | |
| Sequence-based reagent | RNAscope probe- Mm Hand2 | Advanced Cell Diagnostics | Cat.#499821 | |
| Sequence-based reagent | RNAscope probe- Mm Shh | Advanced Cell Diagnostics | Cat.#314361 | |
| Sequence-based reagent | RNAscope probe- Mm Ptch1 | Advanced Cell Diagnostics | Cat.#402811 | |
| Sequence-based reagent | RNAscope probe- Mm Foxd1 | Advanced Cell Diagnostics | Cat.#495501-C3 | |
| Sequence-based reagent | RNAscope probe- Mm Plagl1 | Advanced Cell Diagnostics | Cat.#462941 | |
| Sequence-based reagent | RNAscope probe- Mm Myh6 | Advanced Cell Diagnostics | Cat.#506251 | |
| Sequence-based reagent | RNAscope probe- Mm Gli2 | Advanced Cell Diagnostics | Cat.#405771 | |
| Sequence-based reagent | RNAscope probe- Mm Maf | Advanced Cell Diagnostics | Cat.#412951 | |
| Software, algorithm | Strand NGS | https://www.strand-ngs.com/ | ||
| Software, algorithm | HOMER | PMID:20513432 | RRID:SCR_010881 | |
| Software, algorithm | RELI | PMID:29662164 | ||
| Software, algorithm | COSMO | PMID:25905672 | ||
| Other | Cis-BP | PMID:25215497 | RRID:SCR_017236 | Transcription factor motif library http://cisbp.ccbr.utoronto.ca/index.php |