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. 2020 Oct 14;20(Suppl 1):156. doi: 10.1186/s12870-020-02332-4

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 4 — Expression analysis of five selected HvSAP genes. a HvSAP5; b HvSAP6; c HvSAP11; d HvSAP12; e HvSAP15, in leaves of four barley cultivars grown in hydroponics with 150 mM NaCl solution for 0 (Control), 3, 7 and 14 days. f Averaged expression of the five selected genes in Control plants of four studied barley accessions for comparison. Expression data were normalised using the averages of two reference genes, HvADP and HvGAPDH, and presented as the average ± SE of three biological and two technical replicates for each genotype and treatment. Significant differences are indicated by asterisks compared to Controls within each experiment, and for each barley accession (a-e) and among studied genes (f): * P > 0.95; ** P > 0.99, calculated using ANOVA