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. 2020 Oct 9;6(41):eaaz9124. doi: 10.1126/sciadv.aaz9124

Fig. 6. Interactions between β cells and innervation with development of diabetes in STZ-treated mice.

Fig. 6

(A) Distribution of insulin+ islets located <1.6 and >1.6 μm from the nearest nerve. (B) Mean volume for insulin+ islets ± NF200+ innervation. Islets per group: 10,199/929/5300/366/9837/1022. (C) Total volume for insulin+ islets ± NF200+ innervation. (D) Intensity of NF200 immunolabeling normalized for insulin+ islet volume. Islets per group: 3013/2762/1837/605/2842/1791/1057/336/5800/3274/1500/386. (E) Ganglia per cubic millimeter. (F) Volume of intrapancreatic ganglia. Ganglia per group: 114/73/97. (G) Distance between intrapancreatic ganglia and insulin+ islets. Ganglia per group: 114/73/97. (H) Percentage of β cells contacting nerves per islet. Islets per group: 69/28/69. Data are shown as means ± SEM or as median ± 95% confidence interval where indicated. Analyses by Kruskal-Wallis with Dunn’s test (B to D) for comparison between control, STZ day 5, and STZ day 15, and unpaired t test for comparison between duodenal and splenic pancreas (E to H). *P < 0.05, **P < 0.01, and ***P < 0.001. T, total; D, duodenal; S, splenic. N = 6 control, N = 5 STZ day 5, and N = 5 STZ day 15 (A to D); N = 6 control, N = 3 STZ day 5, and N = 3 STZ day 15 (E to H).