Fig. 2.

MiR-101-3p specifically targets STC1 in goat granulosa cells. a Target sites for miR-101-3p in the STC1 3′-UTR and the construction of the luciferase expression vector (Luc) fused with the STC1 3′-UTR. wt represents the Luc reporter vector with the wild-type STC1 3′-UTR; mu represents the Luc reporter vector with the mutation at the miR-101-3p site in STC1 3′-UTR. b After HEK293T cells transfected miR-101-3p mimics (miR-101-3p-mi) or mimics NC (NC-mi), miR-101-3p inhibitors (miR-101-3p-in) or inhibitors NC (NC-in) with wt-/mu-Luc reporter vectors for 48 h, the relative luciferase activities are measured. The (c) mRNA or (d) protein expressions of STC1 in granulosa cells transfected with miR-101-3p-mi/-in or NC-mi/-in for 24 h or 48 h are quantified using RT-qPCR or Western blot, respectively. β-actin is used as an internal control. Values are expressed as mean ± SD of n = 3. * P < 0.05, ** P < 0.01. NS means no significance