Fig. 3.

MiR-101-3p promotes steroid hormone synthesis. Goat granulosa cells are transfected with miR-101-3p mimics (miR-101-3p-mi) or mimics NC (NC-mi), miR-101-3p inhibitors (miR-101-3p-in) or inhibitors NC (NC-in). a After 24 h, cell-free supernatants are collected and estrogen (E2) and progesterone (P4) secretions are measured in 50 μL supernatants using ELISA kits. The (b) mRNA or (c) protein expressions of steroid hormone synthesis-associated vital genes CYP11A1, CYP19A1, 3β-HSD and STAR are quantified using RT-qPCR or Western blot after 24 or 48 h treatment, respectively. β-actin is used as an internal control. Values are expressed as mean ± SD of n = 3. * P < 0.05, ** P < 0.01. Superscripts (a, b, c) show significant difference. NS means no significance