Fig. 2.
Example of Southern blot analyses of genomic DNAs from PCR- positive T0 transgenic plants of emmer wheat ‘Runo’ generated after biolistic-mediated transformation using IE-derived explants (labeled as PI) and ME-derived explants (labeled as PM); genomic as well as plasmid control DNA was digested with HindIII and the membrane was probed using the 510-bp Ubi1-BAR probe; lane P, psGFP-BAR (1.0 ng); lane WT, negative control representing DNA from untransformed ‘Runo’; under the gel, the inheritance of GFP expression in T1 seedlings of T0 primary plant is indicated