Table 4.
Summary of segregation analysis of GFP expression in T1 progeny (embryos) and seeds (endosperms) of T0 transgenic events of emmer wheat ‘Runo’ (T.dicoccum (Schrank)) a
| Visual GFP expression segregation ratio (positive:negative) | Endosperms | Embryos | Events with the same segregation in embryos and endosperms | |||
|---|---|---|---|---|---|---|
| No. independent T0 events | Percentage | No. independent T0 events | Percentage | No. independent T0 events | Percentage | |
| No. GFP expression | 1 | 1 | 6 | 7 | – | – |
| < 1:1 | 11 | 12 | 17 | 18 | 5 | 5 |
| 1:1 | 19 | 21 | 16 | 17 | 10 | 11 |
| 1:1–3:1 | 15 | 16 | 8 | 9 | 4 | 4 |
| 3:1 | 32 | 35 | 32 | 35 | 26 | 28 |
| 3:1–15:1 | 7 | 8 | 3 | 3 | 1 | 1 |
| 15:1 | 5 | 5 | 8 | 9 | 5 | 5 |
| > 15:1 | 2 | 2 | 2 | 2 | 2 | 2 |
| Total events with the same segregation in embryos and endosperm | 53 | 58 | ||||
| Total No. events analysed | 92 | 92 | 92 | |||
aOnly events with normal fertility are presented, progeny were categorized into the respective classes by chi-square analysis (P > 0.05)