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. 2020 Oct 14;20:507. doi: 10.1186/s12888-020-02903-7

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 2 — Overview of the study schedule. Participants undergo a 30 h protocol, starting at 8:30 am in the morning of day 1 until approximately 1:00 pm of day 2. Study components flow as per Fig. 3. Individuals wear an accelerometer for 29 h of the study. The fasting period commences at 18:30 following dinner on day 1 and continues until 12:30 on day 2. Neurocognitive testing, neuroimaging, biological sampling, and microdialysis are conducted before (T1) and after (T2) the experimental fast