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. 2020 Oct 14;16:89. doi: 10.1186/s13223-020-00487-1

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 5 — VitD deficiency reduces the frequency of Tregs and Bregs in the lungs of the mice. The offspring mice were randomized and injected with PBS or HDM to induce allergic airway inflammation. The percentages of CD4⁺CD25⁺Foxp3⁺ Tregs (a, c) and CD19⁺IL-10⁺ Bregs (b, d) in individual BALF samples were determined by flow cytometry. Data are representative flow cytometry charts and expressed as the mean values of individual mice in each group (n = 8 mice per group) from three separate experiments. *p < 0.05, **p < 0.01, ***p < 0.001

Fig. 5 — VitD deficiency reduces the frequency of Tregs and Bregs in the lungs of the mice. The offspring mice were randomized and injected with PBS or HDM to induce allergic airway inflammation. The percentages of CD4⁺CD25⁺Foxp3⁺ Tregs (a, c) and CD19⁺IL-10⁺ Bregs (b, d) in individual BALF samples were determined by flow cytometry. Data are representative flow cytometry charts and expressed as the mean values of individual mice in each group (n = 8 mice per group) from three separate experiments. *p < 0.05, **p < 0.01, ***p < 0.001