Table 2. PCR Primer Sequences and Product Sizes (Base Pairs)^a.

gene	primer sequence	amplicon size (bp)
ALP	S: 5′-CATGCTGAGTGACACAGACAAGAA-3′	141
	A: 5′-ACAGCAGACTGCGCCTGGTA-3′
OCN	S: 5′-GACGAGTTGGCTGACCACA-3′	138
	A: 5′-CAAGGGGAAGAGGAAAGAAGG-3′
Runx2	S: 5′-TCCACACCATTAGGGACCATC-3′	136
	A: 5′-TGCTAATGCTTCGTGTTTCCA-3′
GAPDH	S: 5′-TGGCACCCAGCACAATGAA-3′	186
	A: 5′-CTAAGTCATAGTCCGCCTAGAAGCA-3′

^aPCR, polymerase chain reaction; ALP, alkaline phosphatase; RUNX2, runt-related transcription factor 2; and OCN, osteocalcin.