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. 2020 Oct 15;12:48. doi: 10.1186/s13099-020-00387-0

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 3 — Upregulation of Notch-1 and IL-6 in the blood of CD patients and the effect of Mycobacterium avium paratuberculosis (MAP) infection on Notch-1 and IL-6 levels. a, b Expression levels of Notch-1 and IL-6 were measured in peripheral leukocytes isolated from CD and healthy controls (n = 4). c Circulating IL-6 levels were measured in the plasma of CD and healthy controls (n = 22). d, e Expression levels of Notch-1 and IL-6 were measured in peripheral leukocytes isolated from MAP positive and MAP negative individuals (n = 4). f Circulating IL-6 levels were measured in the plasma of MAP positive and MAP negative individuals (n = 22). Gene expression levels were measured using RT-PCR. Plasma IL-6 levels were measured using ELISA. Data are shown as mean ± SD, and significant is considered as *P < 0.05