Figure 4.

Conjugation of murine NK cells with the RMA/S cell target in presence of MNPs. NK cells were treated with MNPs (+ APS-MNPs) or left untreated (− APS-MNPs) and incubated with RMA/S target cells for different lengths of time. Representative images of their conjugation at different incubation times, acquired by confocal microscopy (actin (red), microtubules (blue), perforin (green), MNPs (gray)). Scale: 10 μm. Dark-field confocal imaging is described in [78] and conjugation technique in [79].