Figure 3.
Borrelia loads in various tissues after C3H-HeN mice infection followed by disulfiram or doxycycline antibiotics treatment. (A) Antibiotic treatment/borrelia infection schedule: groups of 5 weeks old C3H mice (n = 30) were infected subcutaneously above the shoulders with mid log-phase 105 B. burgdorferi. Infected groups were received intraperitoneal antibiotics doxycycline (n = 5), disulfiram (n = 5) and PBS (n = 5) at two different time points: day-14 post infection and day-21 post infection. Uninfected groups of mice were kept as controls (n = 10). (B) Necropsy at the end of day-21 and day-28, ears, hearts and urinary bladders were collected for determination of the number of Borrelia flab per ul of sample by qPCR. (C) Photomicrographs (40×) of hematoxylin and eosin-stained heart sections; arrows depict the mono nuclear leucocyte infiltrates. Statistics by unpaired t-test with Welch’s correction between drug-treated group versus infected group. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p <0.0001.