Table 1.
In vivo efficacy of drugs against B. burgdorferi in C3H/HeN mice (on day 14).
| No. of Mice Infected | Drug Name | Post 21 Days Ear Culture in BSK Medium | No. of fla-b DNA Copies/Ear | No. of fla-b DNA Copies/Bladder | No. of fla-b DNA Copies/Heart |
|---|---|---|---|---|---|
| 1 | Saline (No drug) | + | 847 | 1761 | 15893 |
| 2 | + | 791 | 1422 | 28683 | |
| 3 | + | 7851 | 1335 | 79512 | |
| 4 | + | 1275 | 1136 | 16256 | |
| 5 | + | 1447 | 1933 | 28125 | |
| 1 | Doxycycline | - | 0 | 0 | 842 |
| 2 | - | 4 | 0 | 12 | |
| 3 | - | 0 | 0 | 0 | |
| 4 | - | 56 | 0 | 1325 | |
| 5 | - | 10 | 0 | 0 | |
| 1 | Disulfiram | - | 73 | 0 | 935 |
| 2 | - | 0 | 1439 | 0 | |
| 3 | - | 0 | 0 | 1044 | |
| 4 | - | 20 | 307 | 1297 | |
| 5 | - | 20 | 89 | 0 |
After 14 days of B. burgdorferi infection, C3H/HeN mice were treated with following drugs once per day for 5 days (Doxycycline—50 mg/kg and Disulfiram—75 mg/kg). The whole DNA was extracted from urinary bladder, ear and heart and further analyzed data by qPCR. (+) Borrelia observed in 21 days post culture of ears in BSK medium. (−) No borrelia observed in 21 days post culture of ears in BSK medium.