Table 2.
In vivo efficacy of drugs against B. burgdorferi in C3H/HeN mice (on day 21).
| No. of Mice Infected | Drug Name | Post 21 Days Ear Culture in BSK Medium | No. of fla-b DNA Copies/Ear | No. of fla-b DNA Copies/Bladder | No. of fla-b DNA Copies/Heart |
|---|---|---|---|---|---|
| 1 | Saline (No drug) | + | 477 | 625 | 447 |
| 2 | + | 509 | 469 | 663 | |
| 3 | + | 412 | 837 | 534 | |
| 4 | + | 835 | 448 | 745 | |
| 5 | + | 753 | 331 | 556 | |
| 1 | Doxycycline | - | 28 | 0 | 0 |
| 2 | - | 5 | 0 | 0 | |
| 3 | - | 14 | 6 | 0 | |
| 4 | - | 15 | 0 | 0 | |
| 1 | Disulfiram | - | 0 | 0 | 0 |
| 2 | - | 21 | 0 | 0 | |
| 3 | - | 0 | 0 | 0 | |
| 4 | - | 51 | 0 | 0 |
After 21 days of B. burgdorferi infection, C3H/HeN mice were treated with following drugs once per day for 5 days (Doxycycline—50 mg/kg and Disulfiram—75 mg/kg). The whole DNA was extracted from urinary bladder, ear and heart and further analyzed data by qPCR.