Figure 1.

(Left) upper and lower, the double plate method. In this example, 3 discs can be inoculated with Af, and 3 different measurements are performed after incubation with a Pseudomonas aeruginosa (Pa) inoculum placed on (in this example) the upper plate. (Middle) upper and lower, the single plate method. Af is demonstrated growing on half the agar, while the other half can be inoculated with Pa. (Right) jar method. A vial containing liquid (in this example) containing a test article can be placed on the lid with an adhesive, whereas a layer of agar in the jar is inoculated with Af on the discs. Alternatively, the lid could contain agar inoculated with Pa and the lid could be screwed on loosely or tightly, depending on the conditions desired.