Figure 4.
Mechanism of inhibition is by the absorption of inhibitory Pa molecules into agar. In the double plate method, TSA was inoculated with Pa10 (40 μL of a 109/mL suspension) (plate #2) and paired with an uninoculated TSA plate (plate #1). An uninoculated TSA plate (plate #3) was paired with another uninoculated plate (plate #4). These pairs were incubated 72 h at 37 °C. Then, plates #2 and 4 were removed, and #1 and 3 were inoculated with 10 μL of a suspension of 5 × 104 10AF conidia, covered, incubated at 37 °C, and the areas of fungal growth calculated. Three asterisks = p < 0.001 compare the growth on plate 1, which was exposed to the aerosol products of Pa, to plate 3, which was exposed to only an uninoculated TSA plate. Times of incubation and measurement are indicated.