Figure 6.

Ganonderic acid extract prevented the activation of cell death molecular pathways. (a) Western blot analysis of STAT3, AKT, p53, ERK (total and phosphorylated) and p21 in whole cell extracts from HaCaT cells cultured for 18 h in the presence of the indicated concentrations of the G. lucidum ethanol extract and H₂O₂ for 6 h: Tubulin was used as a control for protein loading. The panel shows a representative Western blot of three different experiments performed with similar results. (b) Histograms represent mean ± SD in densitometry units of scanned immunoblots from the 3 different experiments (ANOVA, *** p < 0.001, ** p < 0.01 and * p < 0.05).