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. 2020 Sep 25;37(3):253–259. doi: 10.5511/plantbiotechnology.20.0225b

Figure 1. Generation of transgenic Camelina expressing high-speed chimeric myosin XI-2. (A) Wild-type Camelina plants for transformation. (B) Schematic diagram of GFP-fused high-speed chimeric myosin XI-2 construct. F1 and R1, forward and reverse primers for PCR analysis of the homozygous transgenic plants. F2 and R2, forward and reverse primers for RT-PCR to confirm the transcript of GFP-fused high-speed chimeric myosin XI-2 gene. (C) Selection of T1 generation seeds using Murashige and Skoog medium containing 30 µM hygromycin and 250 µM claforan. Bar=1 cm. (D) PCR analysis of two independent homozygous transgenic plants. M, 100 bp ladder; N, non-transgenic plant. (E) RT-PCR analysis of two independent homozygous transgenic plants. Camelina ACTIN11 (CsACT11) was used as an internal control.

Figure 1. Generation of transgenic Camelina expressing high-speed chimeric myosin XI-2. (A) Wild-type Camelina plants for transformation. (B) Schematic diagram of GFP-fused high-speed chimeric myosin XI-2 construct. F1 and R1, forward and reverse primers for PCR analysis of the homozygous transgenic plants. F2 and R2, forward and reverse primers for RT-PCR to confirm the transcript of GFP-fused high-speed chimeric myosin XI-2 gene. (C) Selection of T1 generation seeds using Murashige and Skoog medium containing 30 µM hygromycin and 250 µM claforan. Bar=1 cm. (D) PCR analysis of two independent homozygous transgenic plants. M, 100 bp ladder; N, non-transgenic plant. (E) RT-PCR analysis of two independent homozygous transgenic plants. Camelina ACTIN11 (CsACT11) was used as an internal control.