Figure 6.

pEYS611 preserves the integrity of the outer BRB following light damage. Rat received a single ciliary muscle electrotransfection of pEYS611 (30 µg/eye) on D-3 or left untreated (n = 8 animals/group). Except for unexposed animals (no LID), retinal degeneration was induced on D0 by 24 h of bright light exposure (6500 lux). ZO-1 and albumin staining were performed on D8. (A) Representative images of flat-mounted retina/RPE/choroid ZO-1 staining at a distance of 1500–2000 µm from the ON. Scale bar, 50 µm. (B,C) Albumin into the retina was quantified in the inferior (B) and superior (C) poles of the retina. Percentage of albumin diffusion per retina section is presented as mean ± sem. **** p < 0.0001 using Tukey’s multiple comparisons test versus LID/untreated group.