Table 3.

List of non-conventional techniques for liposomes’ fabrication.

Techniques	Disadvantages	Author (Year)
Supercritical reverse phase evaporation	(1)Semi-continuous processes, meaning that the process cannot be replicated at large scale, for example for the massive fabrication of liposomes for vaccine delivery (2)Encapsulation Efficiency of drugs <60%. Higher than conventional methods, but still too low to obtain a large profitability from the process (3)Low stability, i.e., vesicles are not stable over a long observation time (4)Difficult control of particle size distribution linked to problems of replicability	Otake et al. (2006) [30]
Depressurization of an Expanded Solution into Aqueous Media		Meure et al. (2009) [31]
Depressurization of an Expanded Liquid Organic Solution		Zhao, Tamelli (2015) [32]
Supercritical Anti-Solvent		Lesoin et al. (2011) [33]