Figure 3.

RBD-TMD fusion protein retains antigenic and functional conformation. U-2 OS cells were infected with vesicular stomatitis virus (VSV) expressing either GFP or RBD-TMD (MOI = 0.1). Twenty-four hours post-infection, dot blot analysis were performed, and membranes were probed using (A) RBD antibodies, (B) sera from mice immunized with control or SARS-CoV-2 spike, or (C) a combination of recombinant angiotensin-converting enzyme 2 (ACE2) protein and ACE2 antibody. VSV-G and β-actin indicate equal virus and loading levels, respectively.