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. 2020 Oct 10;22(11):617–629. doi: 10.1016/j.neo.2020.09.004

Fig. 4.

Fig. 4

PRMT5 inhibition induces downregulation of Twist1. (A) Quantitative RT-PCR analysis of EMT transcriptional factor (Twist1, Twist2, Zeb1, Zeb2, SNAI1, SNAI2) mRNA levels in HNSCC cells when PRMT5 knockdown in vitro. Values are mean ± SD from three independent experiments. (Student’s t test). (B) Quantitative RT-PCR analysis of Twist1 mRNA levels in HNSCC when treated with 10 μM EPZ015666 for 4 days in vitro. Values are mean ± SD from three independent experiments. (Student’s t test). (C) Quantitative RT-PCR analysis of E-cadherin and N-cadherin mRNA levels in HNSCC after PRMT5 gain- or loss-of-function in vitro. Values are mean ± SD from three independent experiments. (Student’s t test). (D) Immunoblot of E-cadherin, N-cadherin and Twist1 when PRMT5 gain- or loss-of-function in vitro. (E) Immunoblot of E-cadherin, N-cadherin and Twist1 after treated with 10 μM EPZ015666 for 4 days in vitro.