Fig. 5.

Whole proteome changes upon transfection with Par-4 and miR-200c. (A) Heatmap of the differentially expressed proteins (DEPs) in Mock, miR-200c, GFP, GFP-Par-4 and GFP-Par-4/155A-transfected Panc-1 cells, as identified by RPPA analysis. (B) Venn diagram of DEPs in miR-200c and GFP-Par-4-transfected Panc-1 cells. (C) The bar graph depicts the protein targets that were significantly altered in the miR-200c, GFP-Par-4, and GFP-Par-4/155A-transfected Panc-1 cells. (D) Protein interaction network of the significant DEPs common to miR-200c, GFP-Par-4, and GFP-Par-4/155A-overexpressed samples. The network was built using a String program (http://www.string-db.org/), with confidence greater than 0.5. Every node represents a protein, and the line color indicates the type of interaction evidence. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)