Figure 7.

Exposure to low doses of LRRK2 kinase inhibitor also restored mtDNA damage to basal levels. Healthy control or LRRK2 G2019S patient derived LCLs were treated with either RA334 or RA283 with doses ranging from 1 to 100 nM for 24 h. (A) Treatment with RA334 reversed mtDNA damage in LRRK2 G2019S-patient derived LCLs, with (B) no effect on mtDNA copy number. (C) Similarly, exposure to RA283 reduced LRRK2 G2019S-induced mtDNA damage to healthy control levels. (D) Treatment with RA283 did not change mtDNA copy number. The PCR-based assay was performed in technical triplicate for each biological replicate. (*p < 0.001, determined by one-way ANOVA with a Tukey’s post-hoc comparison). n = 3 biological replicates (6 cell lines total), each performed in technical replicate. Data are presented as mean ± SEM.