Figure 2.

Determination of Stokes radii for human and mouse G0S2 by gel filtration. (A) Analytical SEC chromatogram; the red and blue curves represent the elution profile of human and mouse G0S2, respectively. The inset shows a sodium dodecyl sulfate–polyacrylamide gel electrophoresis of the purified human and mouse G0S2 proteins used in SEC analysis. (B) Calibration curve prepared by plotting the K_AV values of standard proteins (black dots) versus their corresponding R_S value. The red and blue circles correspond to human and mouse G0S2, respectively.