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. 2020 Sep 17;9(9):614. doi: 10.3390/antibiotics9090614

Table 6.

Pimers used in the PCRs carried out for the detection of eight antimicrobial resistance genes in 48 Pasteurella multocida isolates.

Resistance Gene Primer Amplicon Size Annealing Temperature Reference
tetA F: 5′-GTA ATT CTG AGC ACT GTC GC-3′ 1057 pb 62 °C [20]
R: 5′-CTG CCT GGA CAA CAT TGT TT-3′
tetB F: 5′CCT TAT CAT GCC AGT CTT GC-3′ 774 pb 50 °C [20]
R: 5′ ACT GCC GTT TTT TTC GCC-3′
blaROB1 F: 5′ CAT TAA CGG CTT GTT CGC-3′ 852 pb 55 °C [20]
R: 5′-CTT GCT TTG CTG CAT CTT-3′
blaTEM F: 5′GAG TAT TCA ACA TTT TCG T-3′ 856 pb 55 °C [20]
R: 5′-ACC AAT GCT TAA TCA GTG A-3′
ermA F: 5′-ACG ATA TTC ACG GTT TAC CCA CTT-A-3′ 610 pb 53 °C [20]
R: 5-AAC CAG AAA AAC CCT AAA GAC ACG-3′
ermC F: 5′-AAT-CGG CTC AGG AAA AGG-3′ 562 pb 55 °C [20]
R: 5′-ATC GTC ATT TCC TGC ATG-3′
msrE F: 5′-TAT AGC GAC TTT AGC GCC AA-3′ 271 pb 58 °C [20]
R: 3′-GCC GTA GAA TAT GAG CTG AT-3′
mphE F: 5′-ATG CCC AGC ATA TAA ATC GC-3′ 295 pb 58 °C [20]
R: 5′-ATA TGG ACA AAG ATAGCC CG-3′