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. 2020 Oct 14;10:17178. doi: 10.1038/s41598-020-73989-y

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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IL-26 is over-expressed in tuberculous lymph nodes and cutaneous sarcoidosis. (a, c) qPCR analysis of gene expression of IL26, IL22 and IL17A in tuberculous LN (a, n = 12) compared to healthy control LN (n = 9) in RNA from formalin-fixed paraffin-embedded (FFPE) lymph nodes and sarcoidosis skin punch biopsies (c, n = 4–6) compared to healthy skin controls (n = 10). qPCR-values are depicted as relative units compared to 18S RNA expression. Data are presented as single values and mean ± SEM. Mann–Whitney U test was used to evaluate significant differences (*p < 0.05, **p < 0.01 and ***p < 0.001). (b, d) immunohistochemistry with anti-IL-26 and isotype control on FFPE lymph node (LN) sections (10 µm) from one representative tuberculosis patient or healthy control and sections (4 µm) from skin punch biopsies from one representative sarcoidosis patient and one healthy skin donor. Magnification: ×40 for left and right panel, ×100 for middle panel.