Figure 1.

Lack of the Cajal-Retzius cells in the infrapyramidal blade. (A) The infrapyramidal blade (IPB) of dentate gyrus does not develop in Reln^CTRdel, and this coincides with the absence of the RELN-positive cells (gray or red) in the prospective infrapyramidal blade. P7 brains were immunostained with anti-RELN antibodies (n = 2 each). (B) Distribution of p73-expressing cells (gray or green) in the dentate gyrus at P7 (n = 2 each). The number of p73-expressing cells is increased in the suprapyramidal blade (SPB). Nuclei were stained using Hoechst (blue). Scale bars, 250 µm. Arrows indicate the edge of Cajal-Retzius cell-containing regions, which are near the fimbrodentate junction (in the wild type) or the tip of truncated IPB (in the mutants).