Table 4.

Advantages and disadvantages of different locations of expression.

	Advantage	Disadvantage
Cytoplasmic	Higher expression level, simple plasmid construction	Inclusion body may be formed, unfavorable conditions for S-S bond formation, higher proteolysis
Periplasmic	Less proteolysis, improved folding, simple purification	Inclusion body may be formed, the signal does not always facilitate export
Extracellular	Least extensive proteolysis, simpler purification (fewer protein species), improved folding	Usually no secretion is observed, purification may be complex (protein dilution)