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. 2020 Aug 21;12(9):791. doi: 10.3390/pharmaceutics12090791

Figure 4.

Figure 4

(a) Epifluorescence microscopy images: Merged images (bright field (BF) + green fluorescence channel (left)) and green fluorescence channel (right) for HeLa-GFP cells treated with C3(C16His)2/MOG-siRNA lipoplexes (α = 0.2; ρeff = 4 and 10) and Lipo2000*-antiGFP-siRNA as positive control, after 72 h of incubation. Scale bars are 50 μm, except untreated cells which are 10 μm. GFP knockdown levels expressed as MFI (unstriped bars) and % GFP (striped bars) for HeLa-GFP cells at 48 (b) and 72 h (c) of incubation. Cells treated by the lipoplexes were collected at α = 0.2 and ρeff = 4 (light pink bars) and 10 (dark pink bars). Untreated cells were used as a reference of the highest MFI and % GFP value. Free siRNA and Lipo2000*-non-targeting siRNA were used as negative controls (gray and purple bars, respectively) while Lipo2000*-antiGFP-siRNA was used as positive control (blue bars). All experiments were performed with 10% (v/v) of HS. The data represent the mean ± SD of three independent wells.