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Detection of live fluorescence in human mesenchymal stromal cells (hMSCs) treated with PolyCD-based nanoassemblies (2 mg/mL). hMSCs (2 × 104 cells/well in 24-well plates) were directly incubated with the compounds and live fluorescence was monitored after 2 and 24 h by fluorescent microscopy as described in the Materials and Methods (scale bars: 200 µm). Abbreviations: P/DCF: PolyCD@Ada-Rhod/DCF; P: PolyCD@Ada-Rhod. DMEM: Dulbecco’s modified Eagle’s medium.
