Table 1.
Overall properties of PolyCD-based nanoassemblies: mean DH, polidispersity index (PDI) and ζ-potential values (ζ), loading and EE% in ultrapure water.
| Sample | Medium | Mean DH (nm ± SD) a, (%) b | PDI | ζ (mV ± SD) | Theoretical Loading (%) | c Actual Loading (%) | d EE (%) |
|---|---|---|---|---|---|---|---|
| PolyCD | H2O | 268 ± 10 (97) | 0.07 | 19 ± 6 | |||
| PolyCD@Ada-Rhod | H2O | 498 ± 54 (85) | 0.2 | 25 ± 5 | 1.28 (1) | 1.18 ± 0.04 (1) | 92.0 ± 3.3 (1) |
| PolyCD@ AdaRhod/DCF |
H2O | 229 ± 35 (85) 29 ± 13 (12) |
≤0.3 | 22 ± 4 | 1.18 (1) | 1.09 ± 0.05 (1) | 92.0 ± 3.9 (1) |
| 15.5 (2) | 15.5 (2) | ~100 (2) | |||||
| PBS pH 7.4 | 230 ± 24 (83) 25 ± 13 (11) |
≤0.2 | |||||
| NaCl (0.9 wt %) | 228 ± 21 (89) 18 ± 13 (12) |
≤0.2 |
a SD was calculated on three different batches. b Mean size with corresponding intensity % distribution (only main populations). c Actual loading is expressed as the amount of drug (mg) encapsulated per 100 mg of nanoassembly. d Ratio between actual and theoretical loading × 100. (1) Values are referred to Ada-Rhod; (2) Values are referred to Diclofenac; PolyCD (0.5 mg/mL) and PolyCD@Ada-Rhod/DCF (0.5 mg/mL, [Ada-Rhod] = 8 μM, [DCF] = 236 µM).