Figure 5.
Compound 9c potently induces apoptosis in kidney carcinoma cell line A-498 cells (Annexin V/PI FACS). (A) Effect of compound 9c in A-498 cells analyzed by flow cytometry after double staining of the cells with Annexin-V-FITC and PI. A-498 cells treated with 6 µM (approximately IC50 value) of compound 9c for 48 h and collected and processed for analysis. (B) Quantitative analysis of apoptosis. Values represent the mean ± SEM for three independent experiments. Statistical analysis was performed using one-way ANOVA-Bonferroni post-hoc test (**, p < 0.01; ***, p < 0.001). The four quadrants identified as: LL, viable; LR, early apoptotic; UR, late apoptotic; UL, necrotic.