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. 2020 Aug 27;9(9):702. doi: 10.3390/pathogens9090702

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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(a) Immunoblotting of native E. histolytica EF1a to test the specificity of the rabbit anti-eEF1a MAb. Lane 1: standard protein molecular weight marker; Lane 2: trophozoite lysates; Lane 3: native EF1a probed by the rabbit anti-eEF1a MAb from trophozoite lysates. (b) Immunoblot for native E. histolytica EF1a to test specificity of polyclonal antibodies. Lane 1: standard protein molecular weight marker; Lane 2: trophozoite lysates; Lane 3: native EF1a probed by the polyclonal antibodies against rEF1a (dilution of 1:4000) from trophozoite lysates.