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. 2020 Sep 17;10(9):1857. doi: 10.3390/nano10091857

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Biocompatibility assays for patchy magnetite/silver (blue) compared to bare magnetite (black). (*) magnetite cell viability reported elsewhere in the same 72 h and further [36,39]. (A) Cytotoxicity by lactate dehydrogenase enzyme (LDH) in Vero cells line after 24 h. (B) Cytotoxicity by LDH in Vero cells after 48 h. Cell viability remained above 95%. (C) The hemolysis assay shows an average hemolytic effect below 10% in all cases. The positive control was Triton 100-X. (D) Platelet aggregation assay shows an aggregation tendency of about 40%, which is similar to that of bare magnetite. The negative and positive controls were PBS 1X and thrombin, respectively.