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Chromatographic conditions of the gradient HPLC analytical method.
| Time (min) |
Eluent A (%) |
Eluent B (%) |
Flow Rate (mL/min) |
|---|---|---|---|
| 0.01 | 60 | 40 | 1.0 |
| 5.00 | 60 | 40 | 1.0 |
| 6.00 | 50 | 50 | 1.0 |
| 15.00 | 50 | 50 | 1.0 |
Eluent A: Phosphoric acid 1% w/v in water, pH 2.6; Eluent B: Acetonitrile
