Table 8.

Results obtained from the study of robustness of the HPLC method.

Variable	Value	Curcumin Content a (μg/mL)	RSD a (%)	Quercetin Content a (μg/mL)	RSD a (%)
Column temperature (°C)	35	2.53 ± 0.04	1.67	2.54 ± 0.02	0.82
	40	2.50 ± 0.03	1.59	2.50 ± 0.03	1.87
	45	2.51 ± 0.03	1.41	2.49 ± 0.01	0.40
Mobile phase composition (1% phosphoric acid:acetonitrile, v/v; pH 2.6)	35:65/45:55	2.51 ± 0.06	2.39	2.51 ± 0.06	2.58
	40:60/50:50	2.50 ± 0.03	1.59	2.50 ± 0.03	1.87
	45:55/55:45	2.51 ± 0.03	1.31	2.54 ± 0.05	2.09
pH of the mobile phase	2.3	2.50 ± 0.02	0.89	2.49 ± 0.04	1.94
	2.6	2.50 ± 0.03	1.59	2.50 ± 0.03	1.87
	2.9	2.50 ± 0.03	1.25	2.49 ± 0.09	0.04

^a Mean of three replicates