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. 2020 Sep 19;13(9):256. doi: 10.3390/ph13090256

Figure 6.

Figure 6

Formulation affects potency of DOX depending on the BM status. MCF-10A acini with a lowly developed and compromised BM were treated with ACL and DOX in the liposomal formulations and analyzed by the EdU incorporation assay (A). Quantitative analysis indicated an overall significant reduction of proliferation, except for the treatment with PEG-EL-DOX, with a significantly higher reduction when FL-DOX was compared with PEG-EL-DOX (B). Samples treated with PBS served as a control (CTL). This effect was not found when proliferation was investigated in MCF-10A acini with a highly developed and intact BM (hd; B). Association of the liposome dye DiR with the BM could be shown by immunofluorescent staining of collagen IV after treatment of hd-BM acini with FL (C). Scale bars 20 µm. Box plots are shown, statistical evaluation was done using Kruskal–Wallis test, with post-hoc Dunn’s test (***: p < 0.0005).