Table 1.
Species non-specific primer pairs in selected Encephalitozoon spp. and Enterocytozoon bieneusi.
| Species | Sequence (5′→3′) | Primer Designation | Ta | bp | Ref. |
|---|---|---|---|---|---|
| Encephalitozoon spp. and E. bieneusi | -CACCAGGTTGATTCTGCCTGAC- -CCTCTCCGGAACCAAACCCTG- |
PMP1(V1) PMP2 |
60 | Eb 250 Ec 268 Ei 270 Eh 279 |
[67] |
| Encephalitozoon spp. and E. bieneusi | -CACCAGGTTGATTCTGCC- -GTGACGGGCGGTGTCTAC- |
C1(part V1) C2 |
56 | Eb 1 170 Ec 1 190 Eh 1 205 Ei 1 186 |
[45] |
| Encephalitozoon spp. and E. bieneusi | -CACCAGGTTGATTCTGCCTGAC- -GGTTACCTTGTTACGACTT- |
V1 1 492 |
- | around 1200 |
- |
| Encephalitozoon spp. | -TGCAGTTAAAATGTCCGTAGT- -TTTCACTCGCCGCTACTCAG- |
int530f int580r |
40 | around 1000 |
[42] |
| Nested PCR Encephalitozoon spp. and E. bieneusi | -TGAATG(G/T)GTCCCTGT- -TCACTCGCCGCTACT- |
MSP1–PR MSP2A |
58 | - | [49] |
| -GGAATTCACACCGCCCGTC(A/G)(C/T)TAT- -CCAAGCTTATGCTTAAGT(C/T)(A/C)AA(A/G)GGGT- |
MSP3–DR MSP4A |
58 | Ec 289 Ei 305 |
||
| -TGAATG(G/T)GTCCCTGT- -GTTCATCGCACTACT- |
MSP1–PR MSP2B |
58 | − | ||
| -GGAATTCACACCGCCCGTC(A/G)(C/T)TAT- -CCAAGCTTATGCTTAAGTCCAGGGAG- |
MSP3–DR MSP4B |
58 | Eb 508 | ||
| Nested PCR Encephalitozoon spp. and E. bieneusi | -CCAGGUTGATUCTGCCUGACG- -TUACCGCGGCUGCUGGCAC- |
Mic3U–PR Mic421U |
65 | Eb 410 Ec 419 Ei 421 Eh 433 |
[68] |
| -AAGGAGCCTGAGAGATGGCT- -CAATTGCTTCACCCTAAGGTC- -GACCCCTTTGCACTCGCACAC- -TGCCCTCCAGTAAATCACAAC- -CCTCCAATCAATCTCGACTC- |
Mic266–DR Eb379 Ec378 Eh410 Ei395 |
62 | Eb 132 Ec 113 Eh 134 Ei 128 |
||
| Nested PCR | -GGTTGATTCTGCCTGACG- -CTTGCGAGC(G/A)TACTATCC- |
PMicF PMicR |
55 | Eb 779 Enc 779 |
[69] |
| -GGTAATTTGGTCTCTGTGTGTGTG- | EnbF | 57 | Eb 440 | ||
| -CTACACTCCCTATCCGTTC- | EnbR | ||||
| -AGTACGATGATTTGGTTG- -ATCCACAT-ACCA- |
EncepF Aceptac |
Enc 629 | |||
| Real-Time PCR Encephalitozoon spp. | -TGTACACACCGCCCGTCG- -TTTCACTCGCCGCTACTC- |
ecfITSf ecfITSr |
60 | Ec 349 Ei 334 Eh 359 |
[35] |
Ta—primer/template annealing temperature; bp—length of amplicon in base pairs; Eb—Enterocytozoon bieneusi; Ec—Encephalitozoon cuniculi; Ei—Encephalitozoon intestinalis; Eh—Encephalitozoon hellem; Enc—Encephalitozoon.