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. 2020 Jul 22;6(3):114. doi: 10.3390/jof6030114

Table 2.

Species-specific primer pairs in Encephalitozoon spp. and Enterocytozoon sp.

Species Sequence (5′→3′) Primer Designation Ta bp Reference
E. bieneusi -GAAACTTGTCCACTCCTTACG-
-CCATGCACCACTCCTGCCATT-		 EBIEF1
EBIER1		 55 607 [65]
E. bieneusi -CACCAGGTTGATTCTGCCTGAC-
ACTCAGGTGTTATACTCACGTC-		 V1
EB450		 48 353 [58,70]
E. bieneusi–RE1 -CACCAGGTTGATTCTGCCTGAC-
-CAGCATCCACCATAGACAC-		 V1
Mic3		 54 446 [71,72]
E. bieneusi -TCAGTTTTGGGTGTGGTATCGG-
-GCTACCCATACACACATCATTC-		 Eb.gc
Eb.gt		 49 210 [73]
E. bieneusi -GCCTGACGTAGATGCTAGTC-
-ATGGTTCTCCAACTGAAACC-		 EbF
EbR		 55 1294 [74]
E. intestinalis -CACCAGGTTGATTCTGCCTGAC-
-CTCGCTCCTTTACACTCGAA-		 V1
Si500		 58 375 [60]
E. intestinalis -GGGGGTAGGAGTGTTTTTG-
-CAGCAGGCTCCCTCGCCATC-		 3
3		 65 930 [75]
E. intestinalis -TTTCGAGTGTAAGGAGTCGA-
-CCGTCCTCGTTCTCCTGCCCG-		 SINTF1
SINTR		 55 520 [76,77]
E. intestinalis -CACCAGGTTGATTCTGCCTGAC-
-CCTGCCCGCTTCAGAACC-		 V1
Sep1		 55 824 -
E. cuniculi -ATGAGAAGTGATGTGTGTGCG-
-TGCCATGCACTCACAGGCATC-		 ECUNF
ECUNR		 55 549 [56,77]
E. hellem -TGAGAAGTAAGATGTTTAGCA-
-GTAAAAAGACTCTCACACTCA-		 EHELF
EHELR		 55 547 [56]

Ta—primer/template annealing temperature; bp—length of amplicon in base pairs; RE1—possibility of the use of restriction enzymes MnII and DdeI for confirmation of amplicon identity (RFLP).