Table 3.
Primer pairs amplifying rRNA genes of new species or genotypes of Encephalitozoon spp. and Enterocytozoon sp.
| Species | Sequence (5′→3′) | Target Region | Primer Designation | bp | Reference |
|---|---|---|---|---|---|
|
E. bieneusi, E. hellem, E. intestinalis, E. cuniculi, other species |
-ACCAGGTTGATTCTGCCTGAC- -GGTTACCTTGTTACGACTT- |
SSU rRNA | V1 1492 |
1200 | [57,58,59,60] |
|
E. bieneusi, E. hellem, E. intestinalis, E. cuniculi, other species |
-CACCAGGTTGATTCTGCC- -TTATGATCCTGCTAATGGTTC- |
SSU rRN | 18f 1537r |
1300 | [80] |
|
E. intestinalis, E. cuniculi, E. hellem, other species |
-CACCAGGTTGATTCTGCCTGA- -TTATGATCCTGCTAATGGTTCTCCAAC- |
SSU rRNA | Micro-F Micro-R |
1300 | [56,81] |
| E. bieneusi | -CACCAGGTTGATTCTGCCTGA- -CAACTGAAACCTTGTTACGACTT- |
SSU rRNA | Micro-F 1492N4 |
1200 | [81] |
|
E. bieneusi, E. hellem, E. intestinalis, E. cuniculi, other species |
-CACCAGGTTGATTCTGCCTGACG- -TTATGATCCTGCTAATGGTTCTCC- |
SSU rRNA | − − |
1300 | [75] |
|
E. bieneusi, E. hellem, E. intestinalis, E. cuniculi, V. cornae, other species |
-GTGCCAGC(C/A)GCCGCGG- -GGTCCGTGTTTCAAGACGG- |
SSU rRNA, ITS, LSU rRNA | 530f 580r |
1550 1350 1350 1350 1300 |
[59,60,82,83] |
|
E. intestinalis, E. hellem, E. cuniculi, other species |
-TGCAGTTAAAATGTCCGTAGT- -TTTCACTCGCCGCTACTC- |
SSU rRNA, ITS, LSU rRNA | int530f int580r |
1000 1000 1000 |
[75,82] |