Table 4.
Primer sequence of microsatellite and minisatellite loci of Enterocytozoon bieneusi for MLST analysis.
| Locus | Sequence (5′→3′) Primers | Targeted Repeat | Annea-Ling Temp (°C) | bp | GenBank Accession No. (Nucleotide Positions) |
|---|---|---|---|---|---|
| MS-1 | F1, CAAGTTGCAAGTTCAGTGTTTGAA R1, GATGAATATGCATCCATTGATGTT F2, TTGTAAATCGACCAAATGTGCTAT R2, GGACATAAACCACTAATTAATGTAAC |
(TAT)31 (TAG)11 | 58 58 |
843 676 |
ABGB01000003 (63854–64529) |
| MS-2 | F1, GTACAAGATGAAGTTCCTGAGT R1, CATGACATCATTTTACATACACAT F2, GGCCTGATAATAGATCGGATT R2, CAGCATCATCACACGTTCTCA |
(TG)19 | 55 55 |
584 421 |
ABGB01001554 (367–787) |
| MS-3 | F1, CAAGCACTGTGGTTACTGTT R1, AAGTTAGGGCATTTAATAAAATTA F2, GTTCAAGTAATTGATACCAGTCT R2, CTCATTGAATCTAAATGTGTATAA |
(TA)21 | 55 55 |
702 537 |
ABGB01000035 (202–738) |
| MS-4 | F1, GCATATCGTCTCATAGGAACA R1, GTTCATGGTTATTAATTCCAGAA F2, CGAAGTGTACTACATGTCTCT R2, GGACTTTAATAAGTTACCTATAGT |
(TTATTTTTTCCATTTTT CTTCTTCTATTTCCTTTA)9 |
55 55 |
965 885 |
ABGB01000033 (1063–1947) |
| MS-5 | F1, GTCATGATCACCGGCACTTA R1, CTCAAGGATCGTCAAGCTGA F2, GCAGGCTTTGCAGTTGGCTT R2, GTGAAGGAAGCCGTAGCTAA |
(GCGGCTGGTTTCGCAG CAGCGGTTTTAGCAA CTGGCTTC)12 |
55 55 |
882 599 |
ABGB01000169 (546–1144) |
| MS-6 | F1, GAATAGAATGATTCTAGCCATGA R1, CCATATAGCCTTTAAGACCAAA F2, CTTTTCAAGGATGGTTTGAATGA R2, CAAAGGGTACCTCCAATCAAA |
(AT)14 | 55 55 |
706 498 |
ABGB01000562 (660–1157) |
| MS-7 | F1, GTTGATCGTCCAGATGGAATT R1, GACTATCAGTATTACTGATTATAT F2, CAATAGTAAAGGAAGATGGTCA R2, CGTCGCTTTGTTTCATAATCTT |
(TAA)13 | 55 55 |
684 471 |
ABGB01000014 (23807–24277) |
Source: [89].