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. 2020 Aug 24;9(9):534. doi: 10.3390/antibiotics9090534

Table 1.

Primers used for amplification and sequencing of the Escherichia coli genes involved in fosfomycin or nitrofurantoin resistance.

Gene Forward Primer Reverse Primer Amplicon Size (bp) Reference
murA 5′-AAACAGCAGACGGTCTATGG-3′ 5′-CCATGAGTTTATCGACAGAACG-3′ 1542 [9]
glpT 5′-GCGAGTCGCGAGTTTTCATTG-3′ 5′-GGCAAATATCCACTGGCACC-3′ 1785
uhpT 5′-TTTTTGAACGCCCAGACACC-3′ 5′-AGTCAGGGGCTATTTGATGG-3′ 1667
uhpA 5′-GATCGCGGTGTTTTTTCAG-3′ 5′-GATACTCCACAGGCAAAACC-3′ 771
ptsI 5′-GAAAGCGGTTGAACATCTGG-3′ 5′-TCCTTCTTGTCGTCGGAAAC-3′ 1908
cyaA1 5′-AACCAGGCGCGAAAAGTGG-3′ 5′-TGATGGCTGATGATCGACTC-3′ 1559 [9]
This study
cyaA2 5′-AAAGCTCAGCCGTGAACGC-3′ 5′-ACCTTCTGGGATTTGCTGG-3′ 1648
nfsA 5′-ATTTTCTCGGCCAGAAGTGC-3′ 5′-AGAATTTCAACCAGGTGACC-3′ 1036 [5]
nfsB 5′-CTTCGCGATCTGATCAACG-3′ 5′-CAACAGCAGCCTATGATGAC-3′ 923
ribE 5′-AAGGGAAGCAGCGCACGAA-3′ 5′-GGACAACTGCCAGGAGTAGA-3′ 634 This study
fosA3 5′-GCGTCAAGCCTGGCATTT-3′ 5′-GCCGTCAGGGTCGAGAAA-3′ 282 [10]
fosA4 5′-CTGGCGTTTTATCAGCGGTT-3′ 5′-CTTCGCTGCGGTTGTCTTT-3′ 230 [11]
fosA5 5′-TATTAGCGAAGCCGATTTTGCT-3′ 5′-CCCCTTATACGGCTGCTCG-3′ 177
fosA6 5′-GCTACGGTTCAGCTTCCAGA-3′ 5′-CGAGCGTGGCGTTTTATCAG-3′ 242 This study
fosC2 5′-CGTTCCGTGGAGTTCTATAC-3′ 5′-CTTGATAGGGTTTAGACTTC-3′ 334 [8]
oqxA 5′-GACAGCGTCGCACAGAATG-3′ 5′-GGAGACGAGGTTGGTATGGA-3′ 339 [12]
oqxB 5′-CGAAGAAAGACCTCCCTACCC-3′ 5′-CGCCGCCAATGAGATACA-3′ 240